M.Sc. Student – graduate 2017
Currently, my work is centered around profiling the changes in DNA methylation (5mC) and hydroxymethylation (5hmC) that coincide with the transformation to malignancy in HOXA9 immortalized blood progenitor cells. Changes in 5mC and 5hmC will also be profiled in the context of the neomorphic IDH1 R132H mutation and treatment with ascorbic acid (Vitamin C). I have use (hydroxy)methylated DNA immunoprecipiation sequencing ((h)meDIP-seq) and bioinformatic tools to identify and functionally characterize genomic regions that undergo differential modification between any of the aforementioned biological contexts.
Lussier AA, Bodnar TS, Mingay M, Morin AM, Hirst M, Kobor MS, Weinberg J. Prenatal Alcohol Exposure: Profiling Developmental DNA Methylation Patterns in Central and Peripheral Tissues. Front Genet. 2018 Dec 4;9:610. doi: 10.3389/fgene.2018.00610. eCollection 2018.
Matthew Mingay et al. provide a survey of the chromatin landscape of HOXA9-IDH1R132H AML and new findings supporting a role for vitamin C in facilitating the epigenetic remodelling that occurs during differentiation of hematopoietic progenitors (Vitamin C induced epigenomic remodelling in IDH1 mutant acute myeloid leukemia, Leukemia accepted article preview 2 June 2017; doi: 10.1038/ leu.2017.171.)
M Mingay, A Chaturvedi, M Bilenky, Q Cao, L Jackson, T Hui, M Moksa, A Heravi-Moussavi, R K Humphries, M Heuser, M Hirst. Vitamin C induced epigenomic remodelling in IDH1 mutant acute myeloid leukemia. Leukemia accepted article preview 2 June 2017; doi: 10.1038/ leu.2017.171.